Flexible nanohybrid substrates utilizing gold nanocubes/nano mica platelets with 3D lightning-rod effect for highly efficient bacterial biosensors based on surface-enhanced Raman scattering.

In this study, gold nanocubes (AuNCs) were quickly synthesized using the seed-mediated growth method and reduced onto the surface of two-dimensional (2D) delaminated nano mica platelets (NMPs), enabling the development of AuNCs/NMPs nanohybrids with a 3D lightning-rod effect. First, the growth-solution amount can be changed to easily adjust the AuNCs average-particle size within a range of 30-70 nm. The use of the cationic surfactant cetyltrimethylammonium chloride as a protective agent allowed the surface of AuNCs and nanohybrids to be positively charged. Positively charged nanohybrid surfaces presented a good adsorption effect for detecting molecules with negative charges on the surface. Additionally, the NMP surfaces were rich in ionic charges and provided a large specific surface area for stabilizing the growth of AuNCs. Delaminated AuNCs/NMPs nanohybrids can generate a 3D hotspot effect through self-assembly to enhance the Raman signal. Surface-enhanced Raman scattering (SERS) is highly sensitive in detecting adenine biomolecules. Its limit of detection (LOD) and Raman enhancement factor reached 10-9 M and 3.6 × 108, respectively. Excellent reproducibility was obtained owing to the relatively regular arrangement of AuNC particles, and the relative standard deviation (RSD) was 10.7%. Finally, the surface of NMPs was modified by adding the hydrophilic poly(oxyethylene)-diamine (POE2000) and amphiphilic PIB-POE-PIB copolymer at different weight ratios. The adjustment of the surface hydrophilicity and hydrophobicity of AuNCs/NMPs nanohybrids led to better adsorption and selectivity for bacteria. AuNCs/POE/NMPs and AuNCs/PIB-POE-PIB/NMPs were further applied to the SERS detection of hydrophilic Staphylococcus aureus and hydrophobic Escherichia coli, respectively. The SERS-detection results suggest that the LOD of hydrophilic Staphylococcus aureus and hydrophobic Escherichia coli reached 92 CFU mL-1 and 1.6 × 102 CFU mL-1, respectively. The AuNCs/POE/NMPs and AuNCs/PIB-POE-PIB/NMPs nanohybrids had different hydrophilic-hydrophobic affinities, which greatly improved the selectivity and sensitivity for detecting bacteria with different hydrophilicity and hydrophobicity. Therefore, fast, highly selective, and highly sensitive SERS biological-detection results were obtained.

Radiomics-based nomogram guides adaptive de-intensification in locoregionally advanced nasopharyngeal carcinoma following induction chemotherapy.

OBJECTIVES: This study aimed to construct a radiomics-based model for prognosis and benefit prediction of concurrent chemoradiotherapy (CCRT) versus intensity-modulated radiotherapy (IMRT) in locoregionally advanced nasopharyngeal carcinoma (LANPC) following induction chemotherapy (IC). MATERIALS AND METHODS: A cohort of 718 LANPC patients treated with IC + IMRT or IC + CCRT were retrospectively enrolled and assigned to a training set (n = 503) and a validation set (n = 215). Radiomic features were extracted from pre-IC and post-IC MRI. After feature selection, a delta-radiomics signature was built with LASSO-Cox regression. A nomogram incorporating independent clinical indicators and the delta-radiomics signature was then developed and evaluated for calibration and discrimination. Risk stratification by the nomogram was evaluated with Kaplan-Meier methods. RESULTS: The delta-radiomics signature, which comprised 19 selected features, was independently associated with prognosis. The nomogram, composed of the delta-radiomics signature, age, T category, N category, treatment, and pre-treatment EBV DNA, showed great calibration and discrimination with an area under the receiver operator characteristic curve of 0.80 (95% CI 0.75-0.85) and 0.75 (95% CI 0.64-0.85) in the training and validation sets. Risk stratification by the nomogram, excluding the treatment factor, resulted in two groups with distinct overall survival. Significantly better outcomes were observed in the high-risk patients with IC + CCRT compared to those with IC + IMRT, while comparable outcomes between IC + IMRT and IC + CCRT were shown for low-risk patients. CONCLUSION: The radiomics-based nomogram can predict prognosis and survival benefits from concurrent chemotherapy for LANPC following IC. Low-risk patients determined by the nomogram may be potential candidates for omitting concurrent chemotherapy during IMRT. CLINICAL RELEVANCE STATEMENT: The radiomics-based nomogram was constructed for risk stratification and patient selection. It can help guide clinical decision-making for patients with locoregionally advanced nasopharyngeal carcinoma following induction chemotherapy, and avoid unnecessary toxicity caused by overtreatment. KEY POINTS: • The benefits from concurrent chemotherapy remained controversial for locoregionally advanced nasopharyngeal carcinoma following induction chemotherapy. • Radiomics-based nomogram achieved prognosis and benefits prediction of concurrent chemotherapy. • Low-risk patients defined by the nomogram were candidates for de-intensification.

Observation of Free-Boundary-Induced Chiral Anomaly Bulk States in Elastic Twisted Kagome Metamaterials.

Chiral anomaly bulk states (CABSs) can be realized by choosing appropriate boundary conditions in a finite-size waveguide composed of two-dimensional Dirac semimetals, which have unidirectional and robust transport similar to that of valley edge states. CABSs use almost all available guiding space, which greatly improves the utilization of metamaterials. Here, free-boundary-induced CABSs in elastic twisted kagome metamaterials with C_{3v} symmetry are experimentally confirmed. The robust valley-locked transport and complete valley state conversion are experimentally observed. Importantly, the sign of the group velocity near the K and K^{'} points can be reversed by suspending masses at the boundary to manipulate the onsite potential. Moreover, CABSs are demonstrated in nanoelectromechanical phononic crystals by constructing an impedance-mismatched hard boundary. These results open new possibilities for designing more compact, space-efficient, and robust elastic wave macro- and microfunctional devices.

Monolithic Strong Coupling of Topological Surface Acoustic Wave Resonators on Lithium Niobate.

Coherent phonon transfer via high-quality factor (Q) mechanical resonator strong coupling has garnered significant interest. Yet, the practical applications of these strongly coupled resonator devices are largely constrained by their vulnerability to fabrication defects. In this study, topological strong coupling of gigahertz frequency surface acoustic wave (SAW) resonators with lithium niobate is achieved. The nanoscale grooves are etched onto the lithium niobate surface to establish robust SAW topological interface states (TISs). By constructing phononic crystal (PnC) heterostructures, a strong coupling of two SAW TISs, achieving a maximum Rabi splitting of 22 MHz and frequency quality factor product fQm of ≈1.2 × 1013  Hz, is realized. This coupling can be tuned by adjusting geometric parameters and a distinct spectral anticrossing is experimentally observed. Furthermore, a dense wavelength division multiplexing device based on the coupling of multiple TISs is demonstrated. These findings open new avenues for the development of practical topological acoustic devices for on-chip sensing, filtering, phonon entanglement, and beyond.

Focusing Micromechanical Polaritons in Topologically Nontrivial Hyperbolic Metasurfaces.

Vertically stacked multiple atomically thin layers have recently widened the landscape of rich optical structures thanks to these quantum metamaterials or van der Waals (vdW) materials, featuring hyperbolic polaritons with unprecedented avenues for light. Despite their far-reaching implications, most of their properties rest entirely on a trivial band topological origin. Here, a 2D approach is adopted toward a micromechanical vdW analogue that, as a result of engineered chiral and mirror symmetries, provides topologically resilient hyperbolic radiation of mechanical vibrations in the ultrasonic regime. By applying laser vibrometry of the micrometer-sized metasurface, we are able to exhibit the exotic fingerprints of robust hyperbolic radiation spanning several frequencies, which beyond their physical relevance, may enable ultrasonic technologies.

Application of Nanohybrid Substrates with Layer-by-Layer Self-Assembling Properties to High-Sensitivity Surface-Enhanced Raman Scattering Detection.

The present study was conducted to prepare and investigate large-area, high-sensitivity surface-enhanced Raman scattering (SERS) substrates. Organic/inorganic nanohybrid dispersants consisting of an amphiphilic triblock copolymer (hereafter referred to simply as "copolymer") and graphene oxide (GO) were used to stabilize the growth and size of gold nanoparticles (AuNPs). Ion-dipole forces were present between the AuNPs and copolymer dispersants, while the hydrogen bonds between GO and the copolymer prevented the aggregation of GO, thereby stabilizing the AuNP/GO nanohybrids. Transmission electron microscopy (TEM) revealed that the AuNPs had particle sizes of 25-35 nm and a relatively uniform size distribution. The AuNP/GO nanohybrids were deposited onto the glass substrate by using the solution drop-casting method and employed for SERS detection. The self-assembling properties of two-dimensional sheet-like GO led to a regular lamellar arrangement of AuNP/GO nanohybrids, which could be used for the preparation of large-area SERS substrates. Following removal of the copolymer by annealing at 300 °C for 2 h, measurements were obtained under scanning electron microscopy. The results confirmed that 2D GO nanosheets were capable of stabilizing AuNPs, with the final size reaching approximately 40 nm. These AuNPs were adsorbed on both sides of the GO nanosheets. Because the GO nanosheets were merely 5 nm-thick, a good three-dimensional hot-junction effect was generated along the z-axis of the AuNPs. Lastly, the prepared material was used for the SERS detection of rhodamine 6G (R6G), a commonly used highly fluorescent dye. An enhancement factor (EF) of up to 3.5 × 106 was achieved, and the limit of detection was approximately 10-10 M. Detection limits of 10-10 M and < 10-10 M were also observed with the detection of Direct Blue 200 and the biological molecule adenine. It is therefore evident that AuNP/copolymer/GO nanohybrids are large-area flexible SERS substrates that hold great potential in environmental monitoring and biological system detection applications.

Photon-phonon collaboratively pumped laser.

In 1917, Einstein considered stimulated photon emission of electron radiation, offering the theoretical foundation for laser, technically achieved in 1960. However, thermal phonons along with heat creation of non-radiative transition, are ineffective, even playing a detrimental role in lasing efficiency. Here, we realize a photon-phonon collaboratively pumped laser enhanced by heat in a counterintuitive way. We observe a laser transition from phonon-free 1064 nm lasing to phonon-pumped 1176 nm lasing in Nd:YVO4 crystal, associated with the phonon-pumped population inversion under high temperatures. Moreover, an additional temperature threshold (Tth) appears besides the photon-pump power threshold (Pth), and a two-dimensional lasing phase diagram is verified with a general relation ruled by Pth = C/Tth (constant C upon loss for a given crystal), similar to Curie's Law. Our strategy will promote the study of laser physics via dimension extension, searching for highly efficient and low-threshold laser devices via this temperature degree of freedom.

Widely targeted metabolomics reveals the effect of different raw materials and drying methods on the quality of instant tea.

Introduction: Instant teas are particularly rich in tea polyphenols and caffeine and have great potential as food ingredients or additives to improve the quality of food and enhance their nutritional and commercial value. Methods: To determine the relationships between raw material, drying method, and sensory and other quality attributes, instant teas were prepared from three tea varieties, namely black, green and jasmine tea, using two drying methods, namely spray-drying (SD) and freeze-drying (FD). Results: Both the raw tea material and drying method influenced the quality of the finished instant teas. Black tea was quality stable under two drying, while green tea taste deteriorated much after SD. Jasmine tea must be produced from FD due to huge aroma deterioration after SD. FD produced instant tea with higher sensory quality, which was attributed to the lower processing temperature. Chemical compositional analysis and widely targeted metabolomics revealed that SD caused greater degradation of tea biochemical components. The flavonoids content changed markedly after drying, and metabolomics, combined with OPLS-DA, was able to differentiate the three varieties of tea. Instant tea preparations via SD often lost a large proportion of the original tea aroma compounds, but FD minimized the loss of floral and fruity aroma compounds. Changes in the tea flavonoids composition, especially during drying, contributed to the flavor development of instant tea. Discussion: These results will provide an practicle method for high-quality instant tea production through choosing proper raw tea material and lowering down drying temperature with non-thermal technologies like FD.

Coexistence of All-Order Topological States in a Three-Dimensional Phononic Topological Crystalline Insulator.

Classical-wave topological materials lacking intrinsic half-integer spins are less robust while more tunable. Here, we explore a single 3-dimensional phononic topological crystalline insulator that simultaneously exhibits a whole family of first-order quadratic surface, second-order hinge, and third-order corner states within the same bandgap. Such a topological crystalline insulator hosting all-order phases originates from the different topological nature when hierarchically projected onto different facets and lower dimensions, thus free from trivial cladding crystals. Our work offers an ideal platform for either robust wave propagation or localization in on-demand dimensions and may facilitate dimension division multiplexing technology.

Through-Holes Design for Ideal LiNbO3 A1 Resonators.

This paper proposes a method to realize ideal lithium niobate (LiNbO3) A1 resonators. By introducing subwavelength through-holes between the interdigital transducer (IDT) electrodes on the LiNbO3 surface, all unfavorable spurious modes of the resonators can be suppressed completely. It is convenient and valid for various IDT electrode parameters and different LiNbO3 thicknesses. Also, this method does not require additional device fabrication steps. At the same time, these through-holes can greatly reduce the suspended area of the LiNbO3 thin film, thus significantly improving the design flexibility, compactness, mechanical stability, temperature stability, and power tolerance of the resonators (and subsequent filters). It is expected to become an important means to promote the practical application of LiNbO3 A1 filters and even all Lamb waves filters.

Spatiotemporal Acoustic Vortex Beams with Transverse Orbital Angular Momentum.

Recently, the discovery of optical spatiotemporal (ST) vortex beams with transverse orbital angular momentum (OAM) has attracted increasing attention and is expected to extend the research scope and open new opportunities for practical applications of OAM states. The ST vortex beams are also applicable to other physical fields that involve wave phenomena, and here we develop the ST vortex concept in the field of acoustics and report the generation of Bessel-type ST acoustic vortex beams. The ST vortex beams are fully characterized using the scalar approach for the pressure field and the vector approach for the velocity field. We further investigate the transverse spreading effect and construct ST vortex beams with an ellipse-shaped spectrum to reduce the spreading effect. We also experimentally demonstrated the orthogonality relations between ST vortex beams with different charges. Our study successfully demonstrates the versatility of the acoustic system for exploring and discovering spatiotemporally structured waves, inspiring further investigation of exotic wave physics.

Ideal acoustic quantum spin Hall phase in a multi-topology platform.

Fermionic time-reversal symmetry ([Formula: see text])-protected quantum spin Hall (QSH) materials feature gapless helical edge states when adjacent to arbitrary trivial cladding materials. However, due to symmetry reduction at the boundary, bosonic counterparts usually exhibit gaps and thus require additional cladding crystals to maintain robustness, limiting their applications. In this study, we demonstrate an ideal acoustic QSH with gapless behaviour by constructing a global Tf on both the bulk and the boundary based on bilayer structures. Consequently, a pair of helical edge states robustly winds several times in the first Brillouin zone when coupled to resonators, promising broadband topological slow waves. We further reveal that this ideal QSH phase behaves as a topological phase transition plane that bridges trivial and higher-order phases. Our versatile multi-topology platform sheds light on compact topological slow-wave and lasing devices.

Dosiomics Risk Model for Predicting Radiation Induced Temporal Lobe Injury and Guiding Individual Intensity-Modulated Radiation Therapy.

PURPOSE: We aimed to assess the value of dose distribution-based dosiomics and planning computed tomography-based radiomics to predict radiation-induced temporal lobe injury (TLI) and guide individualized intensity modulated radiation therapy. METHODS AND MATERIALS: A total of 5599 nasopharyngeal carcinoma patients were enrolled, including 2503, 1072, 988, and 1036 patients in the training, validation, prospective test, and external test cohorts, respectively. The concordance index (C-index) was used to compare the performance of the radiomics and dosiomics models with that of the quantitative analyses of normal tissue effects in the clinic and Wen's models. The predicted TLI-free survival rates of redesigned simulated plans with the same dose-volume histogram but different dose distributions for same patient in a cohort of 30 randomly selected patients were compared by the Wilcoxon matched-pairs signed-rank test. RESULTS: The radiomics and dosiomics signatures were constructed based on 30 selected computed tomography features and 10 selected dose distribution features, respectively, which were important predictors of TLI-free survival (all P <.001). However, the radiomics signature had a low C-index. The dosiomics risk model combining the dosiomics signature, D1cc, and age had favorable performance, with C-index values of 0.776, 0.811, 0.805, and 0.794 in the training, validation, prospective test, and external test cohorts, respectively, which were better than those of the quantitative analyses of normal tissue effects in the clinic model and Wen's model (all P <.001). The dosiomics risk model can further distinguish patients in a same risk category divided by other models (all P <.05). Conversely, the other models were unable to separate populations classified by the dosiomics risk model (all P > .05). Two simulated plans with the same dose-volume histogram but different dose distributions had different TLI-free survival rates predicted by dosiomics risk model (all P ≤ .002). CONCLUSIONS: The dosiomics risk model was superior to traditional models in predicting the risk of TLI. This is a promising approach to precisely predict radiation-induced toxicities and guide individualized intensity modulated radiation therapy.

Graphene Nanoplatelet/Multiwalled Carbon Nanotube/Polypyrrole Hybrid Fillers in Polyurethane Nanohybrids with 3D Conductive Networks for EMI Shielding.

This work reports the preparation of graphene nanoplatelet (GNP)/multiwalled carbon nanotube (MWCNT)/polypyrrole (PPy) hybrid fillers via in situ chemical oxidative polymerization with the addition of a cationic surfactant, hexadecyltrimethylammonium bromide. These hybrid fillers were incorporated into polyurethane (PU) to prepare GNP/MWCNT/PPy/PU nanohybrids. The electrical conductivity of the nanohybrids was synergistically enhanced by the high conductivity of the hybrid fillers. Furthermore, the electromagnetic interference (EMI) shielding effectiveness (SE) was greatly increased by interfacial polarization between the GNPs, MWCNTs, PPy, and PU. The optimal formulation for the preparation of GNP/MWCNT/PPy three-dimensional (3D) nanostructures was determined by optimization experiments. Using this formulation, we successfully prepared GNP/PPy nanolayers (two-dimensional) that are extensively covered by MWCNT/PPy nanowires (one-dimensional), which interconnect to form GNP/MWCNT/PPy 3D nanostructures. When incorporated into a PU matrix to form a nanohybrid, these 3D nanostructures form a continuous network of conductive GNP-PPy-CNT-PPy-GNP paths. The EMI SE of the nanohybrid is 35-40 dB at 30-1800 MHz, which is sufficient to shield over 99.9% of electromagnetic waves. Therefore, this EMI shielding material has excellent prospects for commercial use. In summary, a nanohybrid with excellent EMI SE performance was prepared using a facile and scalable method and was shown to have great commercial potential.

Ultralow-Power RRAM with a High Switching Ratio Based on the Large van der Waals Interstice Radius of TMDs.

Low power and high switching ratio are the development direction of the next generation of resistive random access memory (RRAM). Previous techniques could not increase the switching ratio while reducing the SET power. Here, we report a method to fabricate low-power and high-switching-ratio RRAM by adjusting the interstice radius (rg) between the van der Waals (vdW) layers of transitional-metal dichalcogenides (TMDs), which simultaneously increases the switching ratio and reduces the SET power. The SET voltage, SET power, switching ratio and endurance of the device are strongly correlated with rg. When the ratio of rg to the radius of the metal ions that form the conductive filaments (rg/rAg+) is near 1, the SET voltage and SET power vertically decrease while the switching ratio vertically rises with increasing rg/rAg+. For the fabricated Ag/[SnS2/poly(methyl methacrylate)]/Cu RRAM with an rg/rAg+ of 1.04, the SET voltage, SET power and switching ratio are 0.14 V, 10-10 W and 106, respectively. After 104 switching cycles and a 104 s retention time, the switching ratio of the device can still be stable above 106. Bending has no influence on the performance of the device when the bending radius is not <2 mm.

Au Nanorods on Carbon-Based Nanomaterials as Nanohybrid Substrates for High-Efficiency Dynamic Surface-Enhanced Raman Scattering.

Gold nanorods (AuNRs) with different aspect ratios were prepared by the seed-mediated growth method and combined with three carbon-based nanomaterials of multiple dimensions (i.e., zero-dimensional (0D) carbon black (CB), one-dimensional (1D) carbon nanotubes (CNTs), and two-dimensional (2D) graphene oxide (GO)). The AuNR/carbon-based nanomaterial hybrids were utilized in dynamic surface-enhanced Raman scattering (D-SERS). First, cetyltrimethylammonium bromide (CTAB) was used to stabilize and coat the AuNRs, enabling them to be dispersed in water and conferring a positive charge to the surface. AuNR/carbon-based nanomaterial hybrids were then formed via electrostatic attraction with the negatively charged carbon-based nanomaterials. Subsequently, the AuNR/carbon-based nanomaterial hybrids were utilized as large-area and highly sensitive Raman spectroscopy substrates. The AuNR/GO hybrids afforded the best signal enhancement because the thickness of GO was less than 5 nm, which enabled the AuNRs adsorbed on GO to produce a good three-dimensional hotspot effect. The enhancement factor (EF) of the AuNR/GO hybrids for the dye molecule Rhodamine 6G (R6G) reached 1 × 107, where the limit of detection (LOD) was 10-8 M. The hybrids were further applied in D-SERS (detecting samples transitioning from the wet state to the dry state). During solvent evaporation, the system spontaneously formed many hotspots, which greatly enhanced the SERS signal. The final experimental results demonstrated that the AuNR/GO hybrids afforded the best D-SERS signal enhancement. The EF value for R6G reached 1.1 × 108 after 27 min, with a limit of detection of 10-9 M at 27 min. Therefore, the AuNR/GO nanohybrids have extremely high sensitivity as molecular sensing elements for SERS and are also very suitable for the rapid detection of single molecules in water quality and environmental management.

Triangular gold nanoplates/two-dimensional nano mica platelets with a 3D lightning-rod effect as flexible nanohybrid substrates for SERS bacterial detection.

Triangular gold nanoplates (TAuNPs) were prepared by a one-step rapid growth method and then reduced and stabilized on two-dimensional nano mica nanoplatelets (NMPs). We also prepared TAuNP/NMP nanohybrids with a three-dimensional lightning-rod effect by oxidative etching. The surface of the delaminated NMPs (only 1 nm thick) is highly charged and can provide a large specific surface area; thus, it can be used as a substrate for the stable growth of gold nanoplates. In addition, by controlling relevant synthesis parameters, the edge length of the TAuNPs can be easily adjusted in the range of 30-90 nm. During reduction of the TAuNPs, the cationic surfactant cetyltrimethylammonium chloride was added as a protective agent to surround the TAuNPs; consequently, the surface was positively charged, which facilitates adsorption for detecting molecules with negative charges. When nanohybrids were used in surface-enhanced Raman spectroscopy (SERS) to detect adenine molecules, the limit of detection concentration was 10-9 M. The Raman enhancement factor was 5.7 × 107, and the relative standard deviation (RSD) was 9.8%. Finally, this method was applied to the biological detection of Staphylococcus aureus, and the surface charge and hydrophilic properties of the material significantly improved the SERS signal of S. aureus. The limit of detection concentration was 102 CFU mL-1, and the RSD was 11.2%. The TAuNP/NMP nanohybrids can provide very rapid and sensitive SERS detection of biomolecules.

A monolithic three-dimensional thermal convective acoustic vector sensor with acoustic-transparent heat sink.

An acoustic vector sensor can directly detect acoustic particle velocity based on the measured temperature difference between closely spaced heated wires. For the detection of velocity in three dimensions, an integrated three-dimensional (3 D) sensor is desired, but it remains challenging in MEMS (Micro-Electro-Mechanical System) manufacturing. Here, a novel monolithic 3 D acoustic vector sensor is proposed, which is constructed using in-plane distributed wires assembled with acoustically transparent heat sink. The planar MEMS structure of the proposed sensor makes it easy to be fabricated and packaged. This work offers a new method for the design of acoustic vector sensors and other thermal convection-based MEMS sensors.

Prediction of Long-Term Survival Outcome by Lymph Node Ratio in Patients of Parotid Gland Cancer: A Retrospective study.

Background: Lymph node ratio (LNR) has been reported to reliably predict cancer-specific survival (CSS) in parotid gland cancer (PGC). Our study was designed to validate the significance of LNR in patients with PGC. Methods: Patients diagnosed with stage I-IV PGC were enrolled from Surveillance Epidemiology and End Results database (SEER, N = 3529), which is the training group, and Sun Yat-sen University Cancer Center database (SYSUCC, N = 99), the validation group. We used X-tile software to choose the optimal cutoff value of LNR; then, univariable and multivariable analyses were performed, assessing the association between LNR and CSS. Results: The optimal cutoff value of LNR was 0.32 by X-tile based on 3529 patients from SEER. Cox proportional hazard regression analysis revealed better CSS for patients with LNR ≤ 0.32 (adjusted hazard ratio [HR] 1.612, 95% confidence interval [95% CI] 1.286-2.019; p < 0.001) compared with patients with LNR > 0.32 in SEER. In the SYSUCC cohort, patients with LNR ≤ 0.32 also had better CSS over patients with LNR > 0.32 (p < 0.001). In N2 and N3 stage groups, patients with LNR ≤ 0.32 had superior CSS outcomes over those with the LNR > 0.32 group, but this benefit was absent in the N1 stage group. Conclusions: In conclusion, the lymph node ratio turned out to be an independent prognostic factor for cancer-specific survival of PGC in this study. This valuable information could help clinicians to evaluate the prognosis of PGC and suggest that adequate lymph node dissection is necessary.